Development of a real-time PCR method for Thalassiosira rotula rapid detection     

HE Shanying  YU Zhigang  MI Tiezhu 《海洋学报(英文版)》2007,26(2):133-139

Gene specific primers and DNA probe were designed based on the sequence of 18S rDNA cloned from the red tide alga Thalassiosira rotula. A real-time fluorescent quantitative PCR (RFQ-PCR) method was developed for quantitative detection of T.rotula. The RFQ-PCR assay data showed that the results obtained with the RFQ-PCR quite good agreement with those with the light microscope (LM) counting method, which suggested that the RFQ-PCR could be a useful method for red tide alga detection.  相似文献   

东海原甲藻的分子鉴定   总被引：4，自引：2，他引：4  

罗立明  胡鸿钧  李夜光  齐雨藻  吕颂辉  耿亚红  邓光 《海洋学报》2006,28(1):127-131

采用nrDNAITS序列及18S序列两种分子标记,对东海原甲藻和美国国家海洋藻种保藏中心(CCMP)的具齿原甲藻进行分子鉴定,结果表明,两者之间序列非常相似,两者的nrDNAITS序列碱基差异值仅为0.002,nrDNA18S序列的碱基差异值为0.000,根据分子数据,东海原甲藻和美国国家海洋藻种保藏中心的具齿原甲藻应为同一个种.从基因库获取原甲藻的另外3个种nrD-NAITS序列和8个种的18S序列,用计算机软件进行分析和构建分子系统树,结果显示18S序列用于原甲藻的分子鉴定过于保守,而nrDNAITS更适合于该种属界定的分子标准.  相似文献   

基于COMGIS的徐州市报业信息管理系统     

武俊红  周兴付  孙久运 《北京测绘》2006,(4):35-38,49

针对目前国内报业信息管理中存在的问题,在分析了当前软件开发技术的基础上,提出了通过引入GIS可以方便地对报社的日常业务信息进行管理和分析。徐州市报业信息管理系统的实现采用了组件式软件开发技术,选择MapX和ADO控件,在VisualBasic集成环境下进行开发,系统结构采用客户端/服务器模式。本文详细介绍了徐州市报业信息系统的基本功能、空间数据库和属性数据库的建立、专题应用模型的设计等。  相似文献   

三峡库区四方碑滑坡稳定性与变形趋势预测   总被引：1，自引：0，他引：1  

肖婷  殷坤龙  杨背背 《中国地质灾害与防治学报》2018,29(1):10-14

三峡水库建成后,库水位周期性涨落和暴雨产生的渗流作用导致大量古滑坡的复活或新滑坡的发生。以库区近水平层状结构的四方碑滑坡为例,依据库水位实际调动,将水位从175 m至145 m不同降速与50年一遇暴雨进行工况组合,计算4种工况下滑坡的稳定性及破坏概率。然后采用Geo-studio软件的Sigma模块对滑坡进行变形模拟,运用R/S分析方法判断滑坡的变形持续性,并结合野外调查情况,综合评价分析四方碑滑坡的稳定性。结果表明:滑坡在各工况下整体均处于基本稳定状态,具有低危险性;变形模拟结果显示滑坡前缘位移最大,与野外调查情况一致;各监测点Hurst指数均介于0.5~1,表明时间序列具有正持续性,在研究的时间限度内滑坡的局部破坏增强,应在汛期加强对滑坡前缘的巡查和预警。  相似文献   

Using climate to relate water discharge and area in modern and ancient catchments     

《Sedimentology》2018,65(4):1378-1389

Models relating sediment supply to catchment properties are important in order to use the geological record to deduce landscape evolution and interplay between tectonics and climate. Water discharge (Q _w) is an important factor in the widely used ‘BQART ’ model, which relates sediment load to a set of measurable catchment parameters. Although many of the factors in this equation may be independently estimated with some degree of certainty in ancient systems, water discharge (Q _w) certainly cannot. An analysis of a world database of modern catchments with 1255 entries shows that the commonly applied equation relating catchment area (A ) to water discharge (Q _w = 0·075A^0·8) does not predict water discharge from catchment area well in many cases (R ² = 0·5 and an error spanning about three orders of magnitude). This is because the method does not incorporate the effect of arid and wet climate on river water discharge. The inclusion of climate data into such estimations is an opportunity to refine these estimates, because generalized estimates of palaeoclimate can often be deduced on the basis of sedimentological data such as palaeosol types, mineralogy and palaeohydraulics. This paper investigates how the relationship between catchment area and river discharge varies with four runoff categories (arid, semi‐arid, humid and wet), which are recognizable in the geological record, and modifies the coefficient and exponent of the above‐mentioned equation according to these classes. This modified model yields improved results in relating discharge to catchment area (R ² = 0·95 and error spanning one order of magnitude) when core, outcrop or regional palaeoclimate reconstruction data are available in non‐arid systems. Arid systems have an inherently variable water discharge, and catchment area is less important as a control due to downstream losses. The model here is sufficient for many geological applications and makes it possible to include variations in catchment humidity in mass‐flux estimates in ancient settings.  相似文献   

里下河地区极端降水时空变化及未来趋势分析     

鞠艳  杨星  王轶虹  王冬梅  齐斐  王志寰 《水文》2023,43(4)

随着全球变暖,极端天气事件逐渐增加,影响着社会经济发展,揭示区域极端降水时空变化对防洪减灾具有重要意义。基于1960—2019年的逐日降水,通过MK趋势检验、小波分析、重标极差以及克里金插值方法,从强度、频率和持续性三方面分析里下河地区的极端降水指数,并进一步探究其未来趋势变化。结果表明：（1）里下河地区多年平均降水为1017.25 mm,呈不显著增加趋势;空间分布差异明显,总体呈自西北向东南逐渐增加的分布。（2）研究区内各站点极端降水指数变化不同,总体而言,极端降水强度、频率均呈现增加趋势,持续性呈减少趋势。（3）极端降水指数变化过程中存在3类尺度的周期性变化,在整个时间尺度上存在3个偏多中心和2个偏少中心。（4）除R10mm和R20mm未来变化趋势与过去趋势相反且呈弱持续性,其他极端降水指数未来变化趋势与过去相一致,且过去总体趋势对未来趋势的影响时间长度在9~16年左右。研究结果为里下河地区科学合理应对气象灾害、合理配置水资源提供依据。  相似文献   

甘肃厂坝-李家沟超大型铅锌矿床成矿金属来源——来自闪锌矿原位S-Pb和Zn同位素证据     

魏然  王义天  胡乔青  黄诗康  窦平  胡文荣 《矿床地质》2022,41(4):722-740

甘肃厂坝-李家沟铅锌矿床位于西秦岭多金属成矿带内的西成矿集区,为矿集区内重要的超大型铅锌矿床。矿体赋存在中泥盆统安家岔组的白云石化大理岩及石英片岩中,其成因认识一直存在争议,主要分歧集中在是同生还是后生。文章对不同成矿阶段的闪锌矿,采用多接收电感耦合等离子体质谱(MC-ICP-MS)测定Zn同位素组成、采用激光剥蚀-多接收电感耦合等离子体质谱(LA-MC-ICP-MS)原位微区分析技术测定S、Pb同位素组成,示踪成矿物质来源,并分析矿物沉淀机制,为深入理解矿床成因提供新的精细证据。研究结果显示,Ⅰ、Ⅱ、Ⅲ三个成矿阶段闪锌矿的δ⁶⁶Zn分别为0.08‰~0.29‰,平均0.20‰;0.19‰~0.37‰,平均0.30‰;0.36‰~0.37‰,平均0.37‰。其中,Ⅰ阶段的闪锌矿δ³⁴S_Ⅰ值为20.9‰~26.1‰,平均24.4‰;Ⅱ阶段的闪锌矿δ³⁴S_Ⅱ值为12.2‰~21.9‰,平均19.1‰;Ⅲ阶段的闪锌矿δ³⁴S_Ⅲ值为18.2‰~24.7‰,平均21.5‰。3个阶段的矿石矿物Pb同位素组成变化不大,²⁰⁶Pb/²⁰⁴Pb为17.922~18.013,²⁰⁷Pb/²⁰⁴Pb为15.567~15.647,²⁰⁸Pb/²⁰⁴Pb为37.990~38.266。δ⁶⁶Zn同位素值显示,成矿金属早期来源于围岩海相碳酸盐岩,由于混合了岩浆热液或者是瑞利分馏作用,在成矿作用中后期δ⁶⁶Zn同位素逐渐上升。δ³⁴S同位素值显示,早期硫源主要为地层中的硫酸盐,中后期的δ³⁴S同位素值降低,可能是成矿流体中岩浆热液中的S^2-成分逐渐增多导致,闪锌矿为硫酸盐通过TSR反应沉淀成矿。Pb同位素指示成矿物质来源于上地壳,并混入了部分古老的变质基底的成分。笔者研究发现,厂坝-李家沟铅锌矿的成矿机制为不同来源的流体混合,随着pH值、成矿流体的温度发生变化而沉淀成矿。  相似文献   

西藏尕尔穷铜金矿床S、Pb同位素地球化学特征——成矿物质来源示踪   总被引：2，自引：0，他引：2  

姚晓峰  唐菊兴  李志军  邓世林  胡正华  张志 《地球学报》2012,33(4):528-536

尕尔穷铜金矿为近几年在班公湖-怒江成矿带西段取得找矿新突破的与斑岩有关的矽卡岩型铜金-破碎带型铜金(铁)矿床。通过对矽卡岩型矿石中主要的金属矿物黄铁矿、黄铜矿的S、Pb同位素特征进行研究,进一步确定矿床成矿物质来源,并结合区域矿产特征对区域成矿规律给出指示。结果显示,两种金属矿物中的δ34S主要分布于-2.9‰～0.5‰之间,平均值为-1.1‰,其频率直方图具有塔式分布特征,具幔源硫特征;矿石的208Pb/204Pb主要分布于38.384～39.134之间,207Pb/204Pb主要分布于15.577～15.725之间,206Pb/204Pb主要分布于18.112～18.615之间,μ值在9.44～9.69之间,其具有上地壳与地幔混合的造山带铅特征。矿床成矿物质主要来自具幔源特征物质和念青唐古拉基底片麻岩。在班怒带西段伴随着晚白垩世南羌塘-三江复合板片与冈底斯-念青唐古拉板片之间弧-陆碰撞,在措勤-申扎火山岩浆弧内形成了与上地幔或初生下地壳重熔并受上地壳物质混染的斑岩-矽卡岩铜金矿成矿系列。  相似文献   

Early Permian extensional shearing of an Ordovician granite: The Saint-Eutrope “C/S-like” orthogneiss (Montagne Noire,French Massif Central)     

Pavel Pitra  Marc Poujol  Jean Van Den Driessche  Jean-Charles Poilvet  Jean-Louis Paquette 《Comptes Rendus Geoscience》2012,344(8):377-384

Dating the magmatic events in the Montagne Noire gneiss dome is a key point to arbitrate between the different interpretations of the Late Carboniferous–Early Permian tectonics in this southern part of the Variscan belt. The Saint-Eutrope orthogneiss crops out along the northern flank of the dome. We show that the protolith of this orthogneiss is an Ordovician granite dated at 455 ± 2 Ma (LA-ICP-MS U-Pb dating on zircon). This age is identical to that previously obtained on the augen orthogneiss of the southern flank, strongly suggesting that both orthogneiss occurrences have the same Ordovician protolith. The Saint-Eutrope orthogneiss experienced intense shearing along the Espinouse extensional detachment at ca. 295 Ma (LA-ICP-MS U-Pb-Th on monazite), an age close to that determined previously on mica by the ³⁹Ar-⁴⁰Ar method and contemporaneous with the emplacement age of the syntectonic Montalet granite farther to the west. This normal sense shearing reworked previous fabrics related to Variscan thrusting that can be still observed in the augen orthogneiss of the southern flank, and is responsible for the spectacular “C/S-like” pattern of the Saint-Eutrope orthogneiss. This work also shows that care is needed when dealing with C/S-type structures, since they can develop not only in syntectonic intrusions, but also in orthogneisses affected by an intense secondary deformation, at decreasing temperature.  相似文献   

Phytoplankton, nutrients and hydrography in the frontal zone between the Southwest Indian Subtropical gyre and the Southern Ocean     

J. F. Read  M. I. Lucas  S. E. Holley  R. T. Pollard 《Deep Sea Research Part I: Oceanographic Research Papers》2000,47(12):2341-2367

A survey was made of the Southwest Indian Ocean frontal region between 30 and 50°E containing the Agulhas Return, Subtropical and Subantarctic Fronts. From CTD, SeaSoar and extracted samples the distribution of nitrate, silicate and chlorophyll a is shown to be strongly linked to the front and water mass structure, varying zonally and meridionally. Surface chlorophyll a concentrations were low to the north and south leaving a band of elevated chlorophyll between the Subtropical and Subantarctic Fronts. The low concentration of chlorophyll a to the north, in Subtropical Water, was clearly due to nitrate limitation. Between the Subtropical and Subantarctic Fronts, where the chlorophyll a concentrations were highest, the surface layer showed silicate depletion limiting diatom growth. South of the Subantarctic Front there were deep extending, low concentrations of chlorophyll a, but despite plentiful supplies of macro-nutrients and a well-stratified surface layer, high concentrations of chlorophyll a were absent. Changes from west to east were associated with the meandering of the Southern Ocean Fronts, especially the Subtropical Front, and their strength and proximity to each other. Concentrations of chlorophyll a peaked where the Agulhas Return, Subtropical and Subantarctic Fronts were in close proximity. Combined frontal structures appear to have particularly pronounced vertical stability and are associated with enhanced upwelling of nutrients and leakage of nutrients across the front. Light levels are high within the shallow stable layer. Such conditions are clearly favourable for biological growth and support the development of larger-celled phytoplankton communities.  相似文献